Monoclonal Antibody Production Services
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With over 20 years experience, AIC is a leader in monoclonal antibody production services. AIC offers a reliable, quick, and economical service for your custom monoclonal antibody needs. Each project is divided into phases, and each phase is started only with client approval. A total of 3-4 mg of protein or a carrier-conjugated peptide is needed for each monoclonal antibody production. For conjugated peptide immunizations, a second conjugate (with a different carrier protein) or free peptide is needed for titer evaluation. Genetic immunization in BALB/c or C57 Black mice is available at a slightly higher cost as indicated below.
A setup fee of $500 is required for each project and is applied to the total cost of the monoclonal antibody production.
3-For-2 Special Pricing: Get two monoclonals for the price of one! Order between now and December 15, 2007 and get two monoclonal antibody production projects for the price of one. This offer applies only to protein antigens (>5 kD) or conjugated peptides.
Phase I: Immunization of five BALB/c mice with protein or conjugated peptide in Titermax or Alum. Determination of serum titers. Four – Eight weeks. $800
Phase II: Fusion of spleen cells from selected mouse with SP2/0 myelomas. Identification of antibody producing colonies by immunoassay. Two weeks. $1200
Phase III: Expansion of positive colonies and retest for antibody production. Cryopreservation (3 ampules) of positive colonies. Two weeks. $1500
Phase IV: Cloning of positive cultures and testing of resulting clones by immunoassay. Expansion and cryopreservation (3 ampules) of positive clones. Three-four weeks. $200
Phase V-a: 30 day, in vitro production run from selected clone using Integra Celline Bioreactor.
With DMEM and 10% FBS $700
With BD Cell MAb Serum Free Medium $800
With BD Cell MAb Animal Component Free Medium $1000
Additional Services:
Cloning, per culture $200
Quantitation of IgG $100
Isotype Determination $150
The use of mice other than BALB/c or of a fusion partner other than SP2/0 may be additional.
Genetic Immunizations: generally six-twelve weeks and additional immunizations are required to obtain serum titers sufficient for fusion. Phase I genetic immunization studies with BABL/c mice and intramuscular or intravenous injections are $1000. For C57/Bl mice, the cost is $1200. The use of a carrier, such as PEI is recommended and is an additional cost. Hydrodynamic injection is also available, but requires a licensing fee.
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